Emily Brookes

Emily Brookes Pitch Perfect is a summer series highlighting some of NZ's best loved campgrounds and the people who keep them running.

Emily Brookes Pūrākaunui Bay Rd begins three kilometres inland from the Catlins coastline from which it takes its name.

Comment Pitch Perfect is a series highlighting some of NZ's best loved campgrounds and the people who keep them running.

Comment Pitch Perfect is a series highlighting some of NZ's best loved campgrounds and the people who keep them running.

Pitch Perfect is a series highlighting some of NZ's best loved campgrounds and the people who keep them running. This week, Emily Brookes heads north to the Kai Iwi Lakes.

Genetic variation and 3D chromatin structure have major roles in gene regulation. Due to challenges in mapping chromatin conformation with haplotype-specific resolution, the effects of genetic sequence variation on 3D genome structure and gene expression imbalance remain understudied. Here, we applied Genome Architecture Mapping (GAM) to a hybrid mouse embryonic stem cell (mESC) line with high density of single-nucleotide polymorphisms (SNPs). GAM resolved haplotype-specific 3D genome structures with high sensitivity, revealing extensive allelic differences in chromatin compartments, topologically associating domains (TADs), long-range enhancer-promoter contacts, and CTCF loops. Architectural differences often coincide with allele-specific differences in gene expression, and with Polycomb occupancy. We show that histone genes are expressed with allelic imbalance in mESCs, and are involved in haplotype-specific chromatin contacts marked by H3K27me3. Conditional knockouts of Polycomb enzymatic subunits, Ezh2 or Ring1, show that one-third of ASE genes, including histone genes, is regulated through Polycomb repression. Our work reveals highly distinct 3D folding structures between homologous chromosomes, and highlights their intricate connections with allelic gene expression.

Brisbane is well known for its sandy spots and sports stars, but there are plenty of different experiences to be had in Queensland's largest city.

Emily Brookes The place The 5-star W Brisbane is perched on the banks of the Brisbane River, in the heart of Queensland's biggest city.

Fame was instantaneous when Peter Andre burst on the scene, all shiny abs and singing about a Mysterious Girl . Fast-forward 30 years and, as he

Your ideal camping experience probably looks something like barbecues, beach swims and soaking up the summer sunshine.