Jeffrey Johnson

This “New Approach” is either a massive failure to plan or an actual plan to fail.

The win marked the first time Cal has defeated a Top 15 team since a 20-19 win at No. 14 Washington on Sept. 7, 2019. The victory over No. 14 Louisville makes the Golden Bears bowl-eligible for the third straight season to mark their' longest streak of bowl-eligibility since eight straight seasons from 2002-09 (played in seven straight bowls from 2003-09). The Bears were bowl-eligible in 2002 but not selected for a bowl.

Orthoflaviviruses are RNA viruses responsible for significant diseases in humans, domesticated animals and wildlife. Their NS5 protein is central in viral replication, functioning both as an RNA-dependent RNA polymerase and a methyltransferase, while also modulating cellular processes, including the interferon response. Although viral replication is cytoplasmic, the NS5 protein of several mosquito-borne orthoflaviviruses cycles between the cytoplasm and the nucleus of infected human cells. However, the nuclear localization and function of NS5 of tick-borne orthoflaviviruses, such as tick-borne encephalitis virus (TBEV), remained poorly understood. Microscopy analysis and cell fractionation revealed that the NS5 protein of TBEV localized to both the cytoplasm and nucleoplasm of infected cells. Mutagenesis studies identified critical residues required for its nuclear targeting. Mutating these residues in a TBEV replicon abolished viral replication. Immunoprecipitation-mass spectrometry analyses performed in two human cell lines infected with TBEV recovered 352 NS5 partners. Among them, 187 were nuclear or partially nuclear. By integrating our interactome data with that of Powassan virus (POWV), another tick-borne orthoflavivirus, we refined a list of 20 high-confidence NS5 partners, including splicing factors and chromatin modulators. Functional analysis revealed that seven of these nuclear partners significantly modulated viral replication, further underscoring the importance of nuclear NS5 in the viral life cycle. Our work advances our understanding of the nuclear function of the NS5 proteins of tick-borne orthoflaviviruses.

U.S. water systems face a $625 billion challenge to repair aging infrastructure, with innovative AI and sensor technologies offering proactive leak detection to save resources and improve water safety.

Precision Stone Design, a destination for luxury stone fabrication and sourcing, announced the grand opening of its 20,000-sq.-ft. Experience Center at 1000 N. Nicholas Blvd. in Elk Grove Village, Ill. This addition complements the company’s existing 20,000-sq.-ft. fabrication workshop and slab warehouse located nearby at 945 Lively Blvd., together forming a dynamic hub for design innovation, technology and craftsmanship in the Chicago area.

As a parent and grandparent, I know firsthand the impact literacy-rich environments have had on our children and grandchildren.

As a retiree, I have the time and the desire to help young children with language and literacy and had the good fortune of finding an organization that helps me do that − Read to Succeed Buffalo.