Yan Wang

Infected diabetic wounds are sustained by a vicious cycle of hyperglycemia-driven bacterial infection, persistent oxidative stress, and excessive inflammation, which collectively disrupt the ordered progression of tissue repair. Here, we engineered a stage-adaptive Janus microneedle patch (MN-FeSAC-PPE) to enable a staged therapeutic process from early antibacterial intervention to subsequent redox–immune microenvironment remodeling and regenerative tissue repair. This stage-adaptive design integrates Fe single-atom nanozymes (Fe-SACs) into the microneedle base to rapidly kill bacteria using near-infrared light, which activates reactive oxygen species (ROS) production, enabling rapid antibacterial activity against wound pathogens. Meanwhile, propolis extract-loaded (PPE) tips deliver antioxidant bioactive compounds into the wound bed to mitigate oxidative stress, modulate the redox-immune microenvironment, and support the inflammatory-to-regenerative transition. In vitro, MN-FeSAC-PPE enhanced antioxidant defense, suppressed pro-inflammatory factors, and protected fibroblasts from oxidative stress-induced mitochondrial dysfunction. Transcriptomic analysis further supported reduced inflammatory signaling and enhanced metabolism-related programs. In

Background Osteoporosis (OP) is a progressive skeletal disorder often exacerbated by pharmacological agents. Proton pump inhibitors (PPIs), particularly esomeprazole, have been epidemiologically linked to OP, but the underlying mechanisms remain unclear. Methods This study mined over 55 million adverse event (AE) records from the FDA Adverse Event Reporting System (FAERS) between 2004 and 2024 to identify drug-related OP signals. Four statistical algorithms (ROR, PRR, BCPNN, EBGM) were applied for signal detection. Logistic regression was used to assess independent risk factors. Esomeprazole, the most frequently reported PPI, was further investigated using a network toxicology framework. Drug-target predictions, differential gene expression analysis, protein-protein interaction network construction, molecular docking and molecular dynamics (MD) simulations were conducted to explore potential molecular hypotheses. Results A total of 52 drugs showed potential OP-related reporting signals. Among PPI-associated OP-related reports, esomeprazole accounted for the largest proportion and was the only PPI with positive signals across all four algorithms. Logistic regression showed that esomeprazole, age, sex, and low body weight were associated with OP-related adverse event reporting. Time-to-onset analysis showed a median latency of 3.5 years. Network toxicology analysis identified 26 overlapping candidate genes between predicted esomeprazole targets and OP-related DEGs. GO and KEGG analyses highlighted bone-related pathways, including MAPK, Notch, and PI3K/AKT signaling. Protein-protein interaction network analysis identified CXCR4, AKT1, and CASP3 as candidate hub genes. Molecular docking and 100-ns MD simulations supported the binding plausibility and conformational stability of the predicted esomeprazole-target complexes. Conclusion This pharmacovigilance and computational toxicology study suggests a notable association between esomeprazole and PPI-associated OP-related adverse event reporting. The identified targets and pathways, including CXCR4, AKT1, and CASP3, provide exploratory mechanistic clues and hypothesis-generating evidence. These findings may support individualized bone health risk assessment in long-term PPI users and provide a basis for future validation studies.

Yonghui Wang MD ∙ Yan Wang MD ∙ Weihao Li MD ∙ Xuemin Zhang MD ∙ Wei Li MD ∙ Tao Zhang MD

Affiliations & Notes Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, United States of America

Scientific Reports , Jul 2026 Wenting Lu Jibo Han Weiwei Lei Xiao Chen Yan Wang Rong Wang Wenting Lu Jibo Han Weiwei Lei

Affiliations & Notes Department of Medical Oncology, Shanghai Chest Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai Key Laboratory of Thoracic Tumor Biotherapy, Shanghai, 200030, China

Frontiers in Plant Science , Jul 2026 Yan Wang Fu Wang Yan Li Shanshan Han Yan Wang Fu Wang Yan Li Shanshan Han

ABSTRACT Objectives Body Roundness Index (BRI) is a new measure of central fat distribution linked to asthma risks, but its connection to asthma in children is unclear.

Frontiers in Nutrition , Jul 2026 Huanhuan Zhao Yan Wang Shaoyang Zhao Huanhuan Zhao Yan Wang Shaoyang Zhao

Background Age-related molecular trajectories of metabolic dysfunction-associated steatotic liver disease (MASLD) remain insufficiently characterized, limiting age-stratified risk assessment and intervention. Objective To test whether MASLD-related molecular changes with age merely reflect accelerated physiological aging or represent a disease-specific divergence. Methods We utilized public transcriptomic data from normal liver and MASLD samples to identify differential transcriptomic signals. We employed Spearman correlation analysis and generalized additive models (GAM) for nonlinear age-related trajectory modeling, and functional enrichment analysis for signaling pathway characterization. Results We integrated 1,354 public liver transcriptome samples from GEO and GTEx databases to construct a cross-age cohort. Differential expression and age-correlation analyses showed minimal overlap (only 22 genes) between age-associated genes in MASLD and controls, with some genes displaying opposite age-related trends. GAM identified four major expression patterns in MASLD—stable, early-life change, late-life acceleration, and mid-life fluctuation—with trajectory inflection time points around ages ~35 and ~75. Functional enrichment indicated that control age-associated genes mainly involved classical cell cycle processes, whereas MASLD changes were enriched for protein deubiquitination, proteostasis imbalance, and p53-related stress and apoptotic signaling. Many MASLD age-associated genes were also associated with fibrosis stage or NAS, largely in concordant directions. Conclusion MASLD exhibits disease-specific age-related transcriptional remodeling characterized by dysregulation of proteostasis and deubiquitination pathways, activation of cellular stress-response programs, and enrichment of p53-associated apoptotic signaling, rather than a simple acceleration of physiological aging.