William C

BOULDER, Colo. — For most of Saturday’s USC-Colorado game, Carl Williams stood at the end of the press box and watched his son, Trojans quarterback Caleb, opera…

Prostate cancer (PCa) has a high prevalence and represents an important health problem, with an increased risk of metastasis. With the advance of CRISPR-Cas9 genome editing, new possibilities have been created for investigating PCa. The technique is effective in knockout oncogenes, reducing tumor resistance. MMP9 and miR-21 target genes are associated with PCa progression; therefore, we evaluated the MMP-9 and miR-21 targets in PCa using the CRISPR-Cas9 system. Single guide RNAs (sgRNAs) of MMP9 and miR-21 sequences were inserted into a PX-330 plasmid, and transfected in DU145 and PC-3 PCa cell lines. MMP9 and RECK expression was assessed by qPCR, WB, and IF. The miR-21 targets, integrins, BAX and mTOR, were evaluated by qPCR. Flow cytometry was performed with Annexin5, 7-AAD and Ki67 markers. Invasion assays were performed with Matrigel. The miR-21 CRISPR-Cas9-edited cells upregulated RECK, MARCKS, BTG2, and PDCD4. CDH1, ITGB3 and ITGB1 were increased in MMP9 and miR-21 CRISPR-Cas9-edited cells. Increased BAX and decreased mTOR were observed in MMP9 and miR-21 CRISPR-Cas9-edited cells. Reduced cell proliferation, increased apoptosis and low invasion in MMP9 and miR-21 edited cells was observed, compared to Scramble. CRISPR-Cas9-edited cells of miR-21 and MMP9 attenuate cell proliferation, invasion and stimulate apoptosis, impeding PCa evolution.

Pursuant to the Copyright Act of 1976, “original works of authorship fixed in any tangible medium of expression, now known or later developed, from…

"Protectionists and isolationists in both chambers have decided to up the ante this year on the NDAA," writes analyst Bill Greenwalt in this new op-ed.

TEMPE, Arizona — The Coach Prime Roadshow rolled into Tempe on Saturday, and if crowd size is any indication, interest/fascination in Deion Sanders’ remade Colo…

From the moment I set foot on the Morgan State campus in August 1968, I never felt unsafe – quite the opposite. During my 4½ years there, Morgan’s campus became…

/PRNewswire/ -- William C. Huff is delighted to unveil the launch of their website as a platform, which showcases their exceptional moving and storage...

Restrictions on entry of scholars will set back U.S. advances

To evaluate the IOP-lowering effect and safety of bimatoprost implant 15 µg compared with selective laser trabeculoplasty in patients with OAG or OHT.

The shift in fluorescence lifetime of tumour tissue with respect to normal tissue after systemic injection of the near-infrared dye indocyanine green can be used to distinguish tumour tissue from normal tissue with high accuracy.