Mohammad Reza

Abstract: This study investigates long-term cardiovascular complications in COVID-19 patients using advanced clustering techniques.

Workplace sexual harassment (WSH) is a global concern among nurses. Understanding nurses' attitudes toward disclosing WSH is essential for addressing this issue.

This study was designed to assess the efficacy of using povidone-iodine (PVI) 10% and levofloxacin 0.5% about one hour before cataract surgery in

Correction to: Scientific Reports https://doi.org/10.1038/s41598-025-91133-6 , Published online 24 February 2025

Y maze test: To evaluate working memory, we assessed spontaneous alternations in Y-maze apparatus.

S100A8 plays a critical role in the pathogenesis of several inflammatory diseases and multiple types of cancer. Therefore, targeting the S100A8 function may alleviate the pathogenic process in various diseases. Here, specific single-chain variable fragment (scFv) antibodies targeting recombinant S100A8 (rS100A8) were selected by phage display technique and characterized using cDNA sequencing, immunoassay tests, and molecular docking. The neutralizing activity of scFvs was examined by a cell viability assay in rS100A8-treated macrophages. Furthermore, the modulatory effects of scFvs on the expression of inflammatory markers and apoptosis-related genes in macrophages and human colorectal carcinoma HT-29 cells treated with rS100A8 or dextran sulfate sodium (DSS) were assessed by RT-qPCR and flow cytometry. Based on our results, four scFvs were identified to be capable of detecting rS100A8 in the immunoassay tests. Among the selected scFvs, two clones (SA8-E6 and SA8-E12), alone or in combination, exhibited the highest blocking activity on rS100A8 and potently inhibited S100A8-induced cytotoxicity in macrophages. The use of a SA8-E6 and SA8-E12 (SA8-E6-12) cocktail inhibited the upregulation of TLR4 and RAGE, as well as inflammation and apoptosis-related genes in macrophages and HT-29 cells stimulated with rS100A8. Additionally, SA8-E6-12 exerted a significant inhibitory effect on inflammation and apoptosis induced by the S100A8/A9 complex in DSS-stimulated macrophages. We also demonstrated by molecular docking that the interaction of S100A8 with SA8-E6-12 was mainly restricted to the binding domain involved in the connection of S100A8 to TLR4. Further studies are required to explore the efficacy of these anti-S100A8 scFvs as potential therapeutic agents using in vivo models of S100A8-driven inflammatory diseases.

Grade IV astrocytoma, also referred to as glioblastoma (GBM), is the most common type of glioma, accounting for over 60% of all brain tumors. It is still a fatal illness in spite of years of investigation and does not currently have a treatment. Thus, scientists and medical professionals are constantly trying to understand the molecular processes and heterogeneity of GBM as well as looking for new ways to improve treatment results. Numerous studies have indicated that nanomaterials, and more especially nanoparticles, offer a great deal of potential for killing cancer cells; as a result, they are being considered as a potential alternative cancer treatment. Several studies have demonstrated that ZnO NPs have shown specific cytotoxicity against cancer cells while leaving normal cells unharmed. In this study we aim to synthesize sodium doped zinc oxide NPs using zingerone in an environmentally friendly manner to evaluate their cytotoxic effects on U87 GBM cell line and normal HEK cell line and investigate the occurrence of apoptosis via apoptosis assay by flowcytometry and gene expression study of TP53 and related genes to apoptosis and cell cycle regulation pathways. It was demonstrated that Na-doped ZnO NPs had a significant cytotoxic effect on U87 cells while having significantly less effect on normal HEK cells. Na-doped ZnO NPs eliminated cancerous cells through apoptosis induction and possibly cell cycle regulation via up-regulation of TP53, PTEN, BAX, P21 and down-regulation of Bcl2. The unique physicochemical properties of nanoparticles turn them into fascinating agents to treat GBM. Hence, the necessity of exploring the vast, yet unknown field of nanoparticles potentials cannot be over looked.

Abstract Background Mitochondrial fission and fusion both contribute to maintaining mitochondrial function and optimizing bioenergetic capacity.

Abstract Numerous interventions have been proposed to reduce the risk of post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis (PEP), but

Background Meningitis during the neonatal period is a potentially devastating condition with high morbidity and mortality. Pathogens cause meningitis varying with gestational age at birth, age at presentation, and geographic location. Methods This 10-year retrospective cross-sectional study, conducted from 2014 to 2023, involved thoroughly evaluating patient files who developed hospital-acquired neonatal meningitis during their hospitalization, with the aim of identifying the causative pathogens and predisposing factors to mortality and hydrocephalus. in a large, referral neonatal intensive care unit at Imam Khomeini Hospital in Ahvaz, Iran. Results The files of 33 infants with meningitis were examined. The number of premature and low birth weight babies was 30(90.9%) and 27(81.8%), respectively. The studied neonates’ mean gestational age and birth weight were 32.6 ± 3.3 weeks and 1812 ± 739 g, respectively. Meningitis with late-onset occurred in 28(84.8%) infants and Gram-negative bacteria in 24(72.7%) infants. Out of a total of 33 neonates with meningitis, 13(39.3%) of whom died. The pathogen most frequently isolated from cerebrospinal fluid cultures was Acinetobacter species, found in 16(48.4%) patients. Occurrences of seizures in the first three days of meningitis, meningitis with gram-negative bacteria, and leukocytosis of more than 15,000 at the onset of meningitis increased the probability of death and hydrocephalus in infants. Conclusion Considering that most of the neonates in this study were premature, had low birth weight, and had nosocomial late-onset meningitis with Gram-negative bacteria, it is necessary to prevent them from contracting meningitis with strict and multifaceted care while hospitalized.