Ana María

Department of Oral and Maxillofacial Surgery, Hospital General Universitario Gregorio Marañón, Universidad Complutense de Madrid, 28007 Madrid, Spain

Department of Agronomy, University of Sevilla, ETSIA, Ctra. Utrera km 1, 41013 Sevilla, Spain

Faculty of Pharmacy and Nutrition, UCAM Universidad Católica San Antonio de Murcia, 30107 Murcia, Spain

Faculty of Pharmacy and Nutrition, UCAM Universidad Católica de Murcia, 30107 Murcia, Spain Author to whom correspondence should be addressed.

The main objective of this study is to evaluate the water quality of different rainwater harvesting (RWH) systems and the social acceptance of their reuse in young users as an exploratory approach. Three RWH systems were implemented, and the quality of harvested rainwater was evaluated focusing on physicochemical and in situ parameters. Social acceptance was studied in one of the RWH systems using an adapted technology acceptance model. An informative talk about the operation of RWH was given to the users, who were students from a rural primary school. Surveys were conducted before and after the talk to evaluate the impact of providing information to users. The social acceptance was studied in one of the RWH systems. The results indicated that the harvested rainwater from RWH systems was suitable for reuse in agriculture despite the increase in turbidity and chloride concentrations in the outlet tap. The concentration of turbidity, phosphate as phosphorus, chloride and nitrate ranged between 0.8 and 1.9 NTU, 0.01–0.2 mg/L, 2.8–5.0 mg/L and 0.3–0.9 mg/L, respectively. In the acceptance study, the survey results and correlation analyses showed that providing information to users is crucial for increasing the acceptance of RWH systems. Moreover, this study demonstrated that RWH systems are a viable alternative technology for reusing and supplying water in arid and semiarid areas.

Department of Plant Production and Protection, NEIKER-Basque Institute of Agricultural Research and Development, Basque Research and Technology Alliance (BRTA), Campus Agroalimentario de Arkaute, 01192 Arkaute, Spain

Open Access Article by Camelia Savulescu Camelia Savulescu SciProfiles Scilit Preprints.org

The search for new synthesis methodologies based on the principles of green chemistry has led to various studies for the production of silver nanoparticles (AgNPs) using extracts from different parts of plants. Based on this, the present study aims to carry out green synthesis (biosynthesis), characterization, and antibacterial evaluation of reduced and stabilized silver nanoparticles (AgNPs) with aqueous extracts of Minthostachys acris in a simple, ecological, and environmentally safe manner. The extraction process of the organic components is performed using two methods: immersion and the agitation of the leaves of Minthostachys acris Schmidt Lebuhn (Muña) at 0.1% for different times (0.5, 1, 3, 6, and 10 min). Compounds such as hydroxycinnamic acid derivatives, quinic, caffeic, rosmarinic acids, and flavonols present in the Muña extract facilitate the formation of AgNPs; this compounds act as a coating and stabilizing agent. The bioactive components from natural resources facilitate the formation of AgNPs, partially or completely replacing the contaminating and toxic elements present in chemical reagents. The biosynthesis is carried out at room temperature for pH 7 and 8. The synthesized AgNPs are characterized by UV-visible spectroscopy to identify the surface plasmon resonance (SPR) band, which shows an absorption peak around 419 nm and 423 nm for pH 7 and p.H 8, respectively, and Fourier-transform infrared spectroscopy (FTIR) to identify the possible biomolecules responsible for bioreduction and stabilization, with a peak at 1634 cm−1. Dynamic light scattering (DLS) shows the hydrodynamic size of the colloidal nanoparticles between 11 and 200 nm, and scanning electron microscopy (SEM) reveals monodisperse AgNPs of different morphologies, mostly nanospheres, while Laser-Induced Breakdown Spectroscopy (LIBS) demonstrates the presence of Ag in the colloidal solution. The evaluation of the bactericidal activity of the AgNPs using the disk diffusion method against Escherichia coli (E. coli) and Staphylococus aureus (S.aureus) shows that the synthesized AgNPs have effective antibacterial activity against E. coli for the extracts obtained at 6 min for both the immersion and agitation methods, respectively. The significance of this work lies in the use of bioactive components from plants to obtain AgNPs in a simple, rapid, and economical way, with potential applications in biomedical fields.

Open Access Article by Ana Belén Peñaherrera-Pazmiño Ana Belén Peñaherrera-Pazmiño SciProfiles Scilit

Background/Objectives: Leptospirosis is a zoonotic disease that is widely distributed around the world and presents symptoms similar to other febrile illnesses in tropical regions, which complicates clinical diagnosis. This study aimed to evaluate the performance and agreement between serological diagnostic tests for detecting both acute and convalescent human leptospirosis, using the micro agglutination test (MAT) as a reference in an endemic region of the Colombian Caribbean. Methods: A prospective descriptive study was conducted on 275 participants with suspected leptospirosis. Paired serum samples were obtained, and an epidemiological survey was conducted. Using the MAT as the gold standard, we calculated positive and negative predictive values, sensitivity, specificity, and kappa index. A Bayesian latent class model was also used to compare the diagnostic tests. Results: In 223 paired serum samples, the sensitivity values for various stages of the disease ranged between 10.8% to 54.1% in the acute and 6.1% to 66.7% during the convalescent phase compared to the MAT. According to the Bayesian model, sensitivity was 9.5% to 75.3% in the acute phase and 5.7% to 85.3% in the convalescent phase. The Kappa value, an indicator of agreement, was moderate for the IgM ELISA in the acute phase (0.553) and substantial in the convalescent phase (0.692). Conclusions: The MAT was the best confirmatory test in both acute and convalescent phases of leptospirosis. Despite the high specificity of ELISA, 21.62% of participants identified as negative by IgM-ELISA in both phases were subsequently confirmed as positive by the MAT. It is necessary to re-evaluate diagnostic guidelines that do not employ the MAT for confirmation and to enhance the diagnostic and clinical identification of leptospirosis within healthcare institutions and public health laboratories while providing a rapid and reliable test for its implementation.